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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [4]
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- Product number
- TA505372 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505372, RRID:AB_2623040
- Product name
- ETS2 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
- Antibody type
- Monoclonal
- Description
- ETS2 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ETS2
- Isotype
- IgG
- Antibody clone number
- OTI1H4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Cardiomyocytes mediate anti-angiogenesis in type 2 diabetic rats through the exosomal transfer of miR-320 into endothelial cells.
Wang X, Huang W, Liu G, Cai W, Millard RW, Wang Y, Chang J, Peng T, Fan GC
Journal of molecular and cellular cardiology 2014 Sep;74:139-50
Journal of molecular and cellular cardiology 2014 Sep;74:139-50
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-ETS2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of ETS2 protein level by using anti-ETS2 antibody in MCECs. Dilution: 1:2000
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ETS2 monoclonal antibody.(1:200)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ETS2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
- Validation comment
- IHC
