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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 693202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#693202, RRID:AB_2632833
- Product name
- Purified anti-ETS2
- Antibody type
- Monoclonal
- Description
- ETS erythroblastosis virus E26 oncogene homologue 2 (ETS2), also known as ETS2IT1, is a member of the ETS transcription factor family. ETS2 contains one conserved winged helix-turn-helix ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T. ETS2 has been found to be an evolutionary conserved proto-oncogene and is a downstream regulator of the Ras/Raf/MAPK pathway. ETS2 participates in the regulation of the genes involved in stem cell development, cell senescence, cell death, apoptosis, and tumorigenesis. Defects in ETS2 gene have been associated with choriocarcinoma and Down syndrome.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- W15163A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human MCF7 cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL of purified anti-ETS2 antibody (clone W15163A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. MW*: protein standards with longer exposure time.
- Conjugate
- Unconjugated
