BS7858

antibody from Bioworld Technology, Inc
Targeting: TOPBP1 Dpb11, KIAA0259, TOP2BP1
Provider product page for BS7858
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
BS7858 - Provider product page
Provider
Bioworld Technology, Inc
Product name
TOPBP1 polyclonal antibody
Antibody type
Polyclonal
Antigen
Recombinant full length Human TOPBP1.
Description
Human DNA topoisomerase II binding protein 1 (TopBP1) contains eight BRCT motifs that are found in proteins regulating the DNA damage response, transcription, and replication. In addition, TopBP1 shares sequence similarity with the fission yeast Rad4/Cut5 protein and the budding yeast DPB11 protein, both of which are required for DNA damage control and/or replication checkpoint control. Phosphorylation of TopBP1 occurs in response to DNA double-strand breaks and replication blocks. TopBP1 forms nuclear foci and localizes to the sites of DNA damage or the arrested replication forks. Downregulation of TopBP1 leads to reduced cell survival, probably due to increased apoptosis. TopBP1 functions as a transcriptional coactivator by enhancing the human papillomavirus (HPV) transcription/replication factor E2. In addition, the HECT-domain ubiquitin ligase, hHYD, cooperates with TopBP1 in DNA damage response. TopBP1 specifically interacts with the C-terminal region of topoisomerase II beta, which suggests a supportive role for TopBP1 in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Isotype
IgG
Vial size
100ul
Concentration
1 mg/ml
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
TOPBP1 protein structure - BS7858 shown in red.