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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [6]
- Immunoprecipitation [1]
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- Product number
- NB100-217 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-217, RRID:AB_2206005
- Product name
- Rabbit Polyclonal TopBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references HUWE1 interacts with PCNA to alleviate replication stress.
A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair.
The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif.
Proteomic identification of PSF and p54(nrb) as TopBP1-interacting proteins.
Neurogenesis requires TopBP1 to prevent catastrophic replicative DNA damage in early progenitors.
Serotype-specific inactivation of the cellular DNA damage response during adenovirus infection.
Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication.
E2F1 localizes to sites of UV-induced DNA damage to enhance nucleotide excision repair.
Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation.
A vertebrate gene, ticrr, is an essential checkpoint and replication regulator.
Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway.
Regulation of p53 by TopBP1: a potential mechanism for p53 inactivation in cancer.
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling.
The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1.
A role for the deubiquitinating enzyme USP28 in control of the DNA-damage response.
TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival.
Choe KN, Nicolae CM, Constantin D, Imamura Kawasawa Y, Delgado-Diaz MR, De S, Freire R, Smits VA, Moldovan GL
EMBO reports 2016 Jun;17(6):874-86
EMBO reports 2016 Jun;17(6):874-86
A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair.
Tomida J, Itaya A, Shigechi T, Unno J, Uchida E, Ikura M, Masuda Y, Matsuda S, Adachi J, Kobayashi M, Meetei AR, Maehara Y, Yamamoto K, Kamiya K, Matsuura A, Matsuda T, Ikura T, Ishiai M, Takata M
Nucleic acids research 2013 Aug;41(14):6930-41
Nucleic acids research 2013 Aug;41(14):6930-41
The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif.
Ohlenschläger O, Kuhnert A, Schneider A, Haumann S, Bellstedt P, Keller H, Saluz HP, Hortschansky P, Hänel F, Grosse F, Görlach M, Pospiech H
Nucleic acids research 2012 Sep 1;40(17):8309-24
Nucleic acids research 2012 Sep 1;40(17):8309-24
Proteomic identification of PSF and p54(nrb) as TopBP1-interacting proteins.
Kuhnert A, Schmidt U, Monajembashi S, Franke C, Schlott B, Grosse F, Greulich KO, Saluz HP, Hänel F
Journal of cellular biochemistry 2012 May;113(5):1744-53
Journal of cellular biochemistry 2012 May;113(5):1744-53
Neurogenesis requires TopBP1 to prevent catastrophic replicative DNA damage in early progenitors.
Lee Y, Katyal S, Downing SM, Zhao J, Russell HR, McKinnon PJ
Nature neuroscience 2012 Jun;15(6):819-26
Nature neuroscience 2012 Jun;15(6):819-26
Serotype-specific inactivation of the cellular DNA damage response during adenovirus infection.
Forrester NA, Sedgwick GG, Thomas A, Blackford AN, Speiseder T, Dobner T, Byrd PJ, Stewart GS, Turnell AS, Grand RJ
Journal of virology 2011 Mar;85(5):2201-11
Journal of virology 2011 Mar;85(5):2201-11
Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication.
Kumagai A, Shevchenko A, Shevchenko A, Dunphy WG
The Journal of cell biology 2011 Jun 13;193(6):995-1007
The Journal of cell biology 2011 Jun 13;193(6):995-1007
E2F1 localizes to sites of UV-induced DNA damage to enhance nucleotide excision repair.
Guo R, Chen J, Zhu F, Biswas AK, Berton TR, Mitchell DL, Johnson DG
The Journal of biological chemistry 2010 Jun 18;285(25):19308-15
The Journal of biological chemistry 2010 Jun 18;285(25):19308-15
Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation.
Blackford AN, Patel RN, Forrester NA, Theil K, Groitl P, Stewart GS, Taylor AM, Morgan IM, Dobner T, Grand RJ, Turnell AS
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 6;107(27):12251-6
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 6;107(27):12251-6
A vertebrate gene, ticrr, is an essential checkpoint and replication regulator.
Sansam CL, Cruz NM, Danielian PS, Amsterdam A, Lau ML, Hopkins N, Lees JA
Genes & development 2010 Jan 15;24(2):183-94
Genes & development 2010 Jan 15;24(2):183-94
Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway.
Liu Y, Fang Y, Shao H, Lindsey-Boltz L, Sancar A, Modrich P
The Journal of biological chemistry 2010 Feb 19;285(8):5974-82
The Journal of biological chemistry 2010 Feb 19;285(8):5974-82
Regulation of p53 by TopBP1: a potential mechanism for p53 inactivation in cancer.
Liu K, Bellam N, Lin HY, Wang B, Stockard CR, Grizzle WE, Lin WC
Molecular and cellular biology 2009 May;29(10):2673-93
Molecular and cellular biology 2009 May;29(10):2673-93
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
Journal of virology 2009 Jan;83(1):117-27
Journal of virology 2009 Jan;83(1):117-27
The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling.
Xu X, Vaithiyalingam S, Glick GG, Mordes DA, Chazin WJ, Cortez D
Molecular and cellular biology 2008 Dec;28(24):7345-53
Molecular and cellular biology 2008 Dec;28(24):7345-53
The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1.
Delacroix S, Wagner JM, Kobayashi M, Yamamoto K, Karnitz LM
Genes & development 2007 Jun 15;21(12):1472-7
Genes & development 2007 Jun 15;21(12):1472-7
A role for the deubiquitinating enzyme USP28 in control of the DNA-damage response.
Zhang D, Zaugg K, Mak TW, Elledge SJ
Cell 2006 Aug 11;126(3):529-42
Cell 2006 Aug 11;126(3):529-42
TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival.
Liu K, Luo Y, Lin FT, Lin WC
Genes & development 2004 Mar 15;18(6):673-86
Genes & development 2004 Mar 15;18(6):673-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TopBP1 Antibody [NB100-217] - WB analysis of TOPBP1 in HeLa cell extract mitotic and interphase. Image courtesy of product review submitted by Jennifer Flynn.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TopBP1 Antibody [NB100-217] - Sample: Whole cell lysate (50 ug for WB) from HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TopBP1 Antibody [NB100-217] - Detection of Mouse. Whole cell lysate from TCMK-1 (15 and 50 ug) cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TopBP1 Antibody [NB100-217] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPBP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TopBP1 Antibody [NB100-217] - Detection of mouse TopBP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TopBP1 antibody NB100-217 (lot NB100-217-2) used for IP at 3 ug per reaction. TopBP1 was also immunoprecipitated by a previous lot of this antibody (lot A300-111A-1). For blotting immunoprecipitated TopBP1, A300-111A was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
