H00003050-M03

antibody from Abnova Corporation

Targeting: HBZ HBZ-T1, HBZ1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003050-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003050-M03, RRID:AB_894113

Product name

HBZ monoclonal antibody (M03), clone 1G10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant HBZ.

Antigen sequence

MSLTKTERTIIVSMWAKISTQADTIGTETLERLFL
SHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDA
VKSIDDIGGAL


Isotype

IgG

Antibody clone number

1G10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HBZ expression in transfected 293T cell line by HBZ monoclonal antibody (M03), clone 1G10.Lane 1: HBZ transfected lysate(15.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HBZ is approximately 10ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to HBZ on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol