NBP2-20460
antibody from Novus Biologicals
Targeting: SP1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-20460 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP1 Antibody [NBP2-20460] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody diluted at 1:2000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP1 Antibody [NBP2-20460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP1 Antibody [NBP2-20460] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SP1 Antibody [NBP2-20460] - Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 ug of OCT1 antibody (NBP2-20460). Western blot analysis was performed using OCT1 antibody (NBP2-20460). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SP1 stained by SP1 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SP1 stained by SP1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: SP1 Antibody [NBP2-20460] - ChIP was performed with 293T chromatin extract and 5 ug of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.