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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-14027 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-14027, RRID:AB_2171049
- Product name
- Anti-SP1
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses.
Transcriptional activation of REST by Sp1 in Huntington's disease models.
Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements.
T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-gamma.
The beta-catenin axis integrates multiple signals downstream from RET/papillary thyroid carcinoma leading to cell proliferation.
Inhibition of the hTERT promoter by the proto-oncogenic protein TAL1
Identification of a cigarette smoke-responsive region in the distal MMP-1 promoter.
Specificity protein-1 and -3 trans-activate the ovine placental lactogen gene promoter.
Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells.
Interferon regulatory factor 5 (IRF5) gene variants are associated with multiple sclerosis in three distinct populations.
STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation.
Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.
Important roles of multiple Sp1 binding sites and epigenetic modifications in the regulation of the methionine sulfoxide reductase B1 (MsrB1) promoter.
Sp1 regulates osteopontin expression in SW480 human colon adenocarcinoma cells.
Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes.
ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.
Shu FJ, Sidell N, Yang D, Kallen CB
The Journal of steroid biochemistry and molecular biology 2010 Jun;120(4-5):172-9
The Journal of steroid biochemistry and molecular biology 2010 Jun;120(4-5):172-9
Transcriptional activation of REST by Sp1 in Huntington's disease models.
Ravache M, Weber C, Mérienne K, Trottier Y
PloS one 2010 Dec 14;5(12):e14311
PloS one 2010 Dec 14;5(12):e14311
Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements.
Mason CE, Shu FJ, Wang C, Session RM, Kallen RG, Sidell N, Yu T, Liu MH, Cheung E, Kallen CB
Nucleic acids research 2010 Apr;38(7):2355-68
Nucleic acids research 2010 Apr;38(7):2355-68
T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-gamma.
Yu Q, Sharma A, Oh SY, Moon HG, Hossain MZ, Salay TM, Leeds KE, Du H, Wu B, Waterman ML, Zhu Z, Sen JM
Nature immunology 2009 Sep;10(9):992-9
Nature immunology 2009 Sep;10(9):992-9
The beta-catenin axis integrates multiple signals downstream from RET/papillary thyroid carcinoma leading to cell proliferation.
Castellone MD, De Falco V, Rao DM, Bellelli R, Muthu M, Basolo F, Fusco A, Gutkind JS, Santoro M
Cancer research 2009 Mar 1;69(5):1867-76
Cancer research 2009 Mar 1;69(5):1867-76
Inhibition of the hTERT promoter by the proto-oncogenic protein TAL1
J-M Terme, V Mocquet, A-S Kuhlmann, L Zane, F Mortreux, E Wattel, M Duc Dodon, P Jalinot
Leukemia 2009 Jul;23(11):2081-2089
Leukemia 2009 Jul;23(11):2081-2089
Identification of a cigarette smoke-responsive region in the distal MMP-1 promoter.
Mercer BA, Wallace AM, Brinckerhoff CE, D'Armiento JM
American journal of respiratory cell and molecular biology 2009 Jan;40(1):4-12
American journal of respiratory cell and molecular biology 2009 Jan;40(1):4-12
Specificity protein-1 and -3 trans-activate the ovine placental lactogen gene promoter.
Jeckel KM, Limesand SW, Anthony RV
Molecular and cellular endocrinology 2009 Aug 13;307(1-2):118-24
Molecular and cellular endocrinology 2009 Aug 13;307(1-2):118-24
Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells.
Wang C, Yu J, Kallen CB
PloS one 2008;3(10):e3523
PloS one 2008;3(10):e3523
Interferon regulatory factor 5 (IRF5) gene variants are associated with multiple sclerosis in three distinct populations.
Kristjansdottir G, Sandling JK, Bonetti A, Roos IM, Milani L, Wang C, Gustafsdottir SM, Sigurdsson S, Lundmark A, Tienari PJ, Koivisto K, Elovaara I, Pirttilä T, Reunanen M, Peltonen L, Saarela J, Hillert J, Olsson T, Landegren U, Alcina A, Fernández O, Leyva L, Guerrero M, Lucas M, Izquierdo G, Matesanz F, Syvänen AC
Journal of medical genetics 2008 Jun;45(6):362-9
Journal of medical genetics 2008 Jun;45(6):362-9
STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation.
Liu X, Vorontchikhina M, Wang YL, Faiola F, Martinez E
Molecular and cellular biology 2008 Jan;28(1):108-21
Molecular and cellular biology 2008 Jan;28(1):108-21
Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.
Ma W, Horvath GC, Kistler MK, Kistler WS
Biology of reproduction 2008 Aug;79(2):289-300
Biology of reproduction 2008 Aug;79(2):289-300
Important roles of multiple Sp1 binding sites and epigenetic modifications in the regulation of the methionine sulfoxide reductase B1 (MsrB1) promoter.
De Luca A, Sacchetta P, Nieddu M, Di Ilio C, Favaloro B
BMC molecular biology 2007 May 22;8:39
BMC molecular biology 2007 May 22;8:39
Sp1 regulates osteopontin expression in SW480 human colon adenocarcinoma cells.
Takami Y, Russell MB, Gao C, Mi Z, Guo H, Mantyh CR, Kuo PC
Surgery 2007 Aug;142(2):163-9
Surgery 2007 Aug;142(2):163-9
Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes.
Chen GY, Osada H, Santamaria-Babi LF, Kannagi R
Proceedings of the National Academy of Sciences of the United States of America 2006 Nov 7;103(45):16894-9
Proceedings of the National Academy of Sciences of the United States of America 2006 Nov 7;103(45):16894-9
ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.
Zhang X, Diab IH, Zehner ZE
Nucleic acids research 2003 Jun 1;31(11):2900-14
Nucleic acids research 2003 Jun 1;31(11):2900-14
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 81
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
