PAB28496

antibody from Abnova Corporation

Targeting: SP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28496

Provider

Abnova Corporation

Product name

SP1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SP1

Antigen sequence

QVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQT
SSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQ
TINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQL
GLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR
REACTCP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with SP1 polyclonal antibody at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with SP1 polyclonal antibody (Cat # PAB28496) at 1-4 ug/ml shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with SP1 polyclonal antibody (Cat # PAB28496) shows strong nuclear positivity in trophoblastic and decidual cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)