Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-27243 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Reduced Thiamine Availability and Hyperglycemia Impair Thiamine Transport in Renal Glomerular Cells through Modulation of Thiamine Transporter 2.
Thiamine transporter 2 is involved in high glucose-induced damage and altered thiamine availability in cell models of diabetic retinopathy.
Mazzeo A, Barutta F, Bellucci L, Trento M, Gruden G, Porta M, Beltramo E
Biomedicines 2021 Apr 5;9(4)
Biomedicines 2021 Apr 5;9(4)
Thiamine transporter 2 is involved in high glucose-induced damage and altered thiamine availability in cell models of diabetic retinopathy.
Beltramo E, Mazzeo A, Lopatina T, Trento M, Porta M
Diabetes & vascular disease research 2020 Jan-Feb;17(1):1479164119878427
Diabetes & vascular disease research 2020 Jan-Feb;17(1):1479164119878427
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SP1 Polyclonal Antibody (Product # PA5-27243). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2. 5% SDS PAGE. SP1 Polyclonal Antibody (Product # PA5-27243) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Localization and expression of thiamine transporters and Sp1 inside renal glomerular cells in physiological conditions. ( a ) Immunofluorescence with Ab anti-THTR1, anti-THTR2, and anti-Sp1 in HPTEC, HPC, HGEC, and HMC (green). Nuclei are counterstained with DAPI (blue). Magnification 400x. ( b ) Relative expression of THTR-1, THTR2, and Sp1 in the 4 cell types. Mean of 5 experiments +- SD.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Expression of thiamine transporters and Sp1 in retinal cells in physiological conditions: comparison of mRNA (a) and protein expression (b) in the different cell types. (c-k) Localization of thiamine transporters and Sp1 inside retinal cells in physiological conditions. Immunofluorescence with Ab (c, f, i) anti-THTR1, (d, g, j) anti-THTR2 and (e, h, k) anti-Sp1 in (c-e) HMEC, (f-h) HRP and (i-k) MIO-M1 cells (green). Nuclei are counterstained with DAPI (blue). Magnification 400x.