HPA006057

antibody from Atlas Antibodies

Targeting: ELF2 EU32, NERF, NERF-1A, NERF-1B, NERF-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA006057

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA006057, RRID:AB_1078733

Product name

Anti-ELF2

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

MTSAVVDSGGTILELSSNGVENQEESEKVSEYPAV
IVEPVPSARLEQGYAAQVLVYDDETYMMQDVAEEQ
EVETENVETVEASVHSSNAHCTDKTIEAAEALLHM
ESPTCLRDSRSPEFIHAAMRPDVITETVVEVSTEE
SEP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, lymph node, placenta and testis using Anti-ELF2 antibody HPA006057 (A) shows similar protein distribution across tissues to independent antibody HPA071166 (B).

Antibody #2 product nr

HPA071166

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-ELF2 antibody HPA006057.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-ELF2 antibody HPA006057.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-ELF2 antibody HPA006057.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta using Anti-ELF2 antibody HPA006057.

Sample type

HUMAN