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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
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- Product number
- MAB3925 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nrf2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Nrf2 in Western blots. In Western blots, no cross-reactivity with mouse and rat Nrf2 and human Nrf1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16236- Isotype
- IgG
- Antibody clone number
- 383727
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Long-term administration of fatty acid amide hydrolase inhibitor (URB597) to rats with spontaneous hypertension disturbs liver redox balance and phospholipid metabolism.
Antioxidant treatment induces reductive stress associated with mitochondrial dysfunction in adipocytes.
Royal jelly attenuates cadmium-induced nephrotoxicity in male mice.
The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension.
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Histone H2AX deficiency causes neurobehavioral deficits and impaired redox homeostasis.
Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis.
Hepatoprotective effect of calculus bovis sativus on nonalcoholic fatty liver disease in mice by inhibiting oxidative stress and apoptosis of hepatocytes.
Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke.
Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions.
Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8.
Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.
The drug monosodium luminol (GVT) preserves crypt-villus epithelial organization and allows survival of intestinal T cells in mice infected with the ts1 retrovirus.
Biernacki M, Ambrożewicz E, Gęgotek A, Toczek M, Skrzydlewska E
Advances in medical sciences 2019 Mar;64(1):15-23
Advances in medical sciences 2019 Mar;64(1):15-23
Antioxidant treatment induces reductive stress associated with mitochondrial dysfunction in adipocytes.
Peris E, Micallef P, Paul A, Palsdottir V, Enejder A, Bauzá-Thorbrügge M, Olofsson CS, Wernstedt Asterholm I
The Journal of biological chemistry 2019 Feb 15;294(7):2340-2352
The Journal of biological chemistry 2019 Feb 15;294(7):2340-2352
Royal jelly attenuates cadmium-induced nephrotoxicity in male mice.
Almeer RS, AlBasher GI, Alarifi S, Alkahtani S, Ali D, Abdel Moneim AE
Scientific reports 2019 Apr 9;9(1):5825
Scientific reports 2019 Apr 9;9(1):5825
The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension.
Biernacki M, Łuczaj W, Jarocka-Karpowicz I, Ambrożewicz E, Toczek M, Skrzydlewska E
Molecules (Basel, Switzerland) 2018 Sep 14;23(9)
Molecules (Basel, Switzerland) 2018 Sep 14;23(9)
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H
Molecular medicine reports 2018 Aug;18(2):1987-1994
Molecular medicine reports 2018 Aug;18(2):1987-1994
Histone H2AX deficiency causes neurobehavioral deficits and impaired redox homeostasis.
Weyemi U, Paul BD, Snowman AM, Jailwala P, Nussenzweig A, Bonner WM, Snyder SH
Nature communications 2018 Apr 18;9(1):1526
Nature communications 2018 Apr 18;9(1):1526
Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis.
Kontostathi G, Zoidakis J, Makridakis M, Lygirou V, Mermelekas G, Papadopoulos T, Vougas K, Vlamis-Gardikas A, Drakakis P, Loutradis D, Vlahou A, Anagnou NP, Pappa KI
BioMed research international 2017;2017:4180703
BioMed research international 2017;2017:4180703
Hepatoprotective effect of calculus bovis sativus on nonalcoholic fatty liver disease in mice by inhibiting oxidative stress and apoptosis of hepatocytes.
He W, Xu Y, Zhang C, Lu J, Li J, Xiang D, Yang J, Chang M, Liu D
Drug design, development and therapy 2017;11:3449-3460
Drug design, development and therapy 2017;11:3449-3460
Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke.
Lin J, Xu F, Wang G, Kong L, Luo Q, Lv Y, Liu J, Wei Y, Li L, Zhang H, Dong J
Evidence-based complementary and alternative medicine : eCAM 2016;2016:1698379
Evidence-based complementary and alternative medicine : eCAM 2016;2016:1698379
Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions.
Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, Schnütgen F, Scheibe S, Seimetz M, Jaksch P, Hyman A, Weissmann N, von Melchner H
The Journal of biological chemistry 2015 Apr 10;290(15):9738-52
The Journal of biological chemistry 2015 Apr 10;290(15):9738-52
Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8.
Toosi S, Orlow SJ, Manga P
The Journal of investigative dermatology 2012 Nov;132(11):2601-9
The Journal of investigative dermatology 2012 Nov;132(11):2601-9
Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.
Cross SA, Cook DR, Chi AW, Vance PJ, Kolson LL, Wong BJ, Jordan-Sciutto KL, Kolson DL
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5015-25
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5015-25
The drug monosodium luminol (GVT) preserves crypt-villus epithelial organization and allows survival of intestinal T cells in mice infected with the ts1 retrovirus.
Scofield VL, Yan M, Kuang X, Kim SJ, Wong PK
Immunology letters 2009 Feb 21;122(2):150-8
Immunology letters 2009 Feb 21;122(2):150-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
