PA5-51659

antibody from Invitrogen Antibodies

Targeting: NFE2 NF-E2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51659

Provider

Invitrogen Antibodies

Product name

NFE2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SRNRVIQLST SELGEMELTW QEIMSITELQ GLNAPSEPSF EPQAPAPYLG PPPPTTYCPC SIHPDSGFPL PPPPYELPAS TSHVPDPPYS YGNMAIPVSK PLSLSGLLSE PLQDPLALLD IGLPAGPPKP QEDPES

Concentration

0.05 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFE2 in human cell line HEL using a NFE2 Polyclonal Antibody (Product # PA5-51659).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFE2 in control (vector only transfected HEK293T lysate) and NFE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a NFE2 Polyclonal Antibody (Product # PA5-51659).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NFE2 in human cell line U-251 MG shows positivity in actin filaments & nucleus but excluded from the nucleoli. Samples were probed using a NFE2 Polyclonal Antibody (Product # PA5-51659).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NFE2 in human bone marrow using a NFE2 Polyclonal Antibody (Product # PA5-51659) shows moderate nuclear positivity in hematopoietic cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NFE2 in human testis using NFE2 Polyclonal Antibody (Product # PA5-51659) shows moderate nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NFE2 in human bone marrow using NFE2 Polyclonal Antibody (Product # PA5-51659) shows moderate to strong nuclear positivity in hematopoietic cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NFE2 in human liver using NFE2 Polyclonal Antibody (Product # PA5-51659) shows no nuclear positivity in hepatocytes as expected.