13025-1-AP

antibody from Invitrogen Antibodies

Targeting: LCP1 CP64, L-PLASTIN, LC64P, PLS2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13025-1-AP

Provider

Invitrogen Antibodies

Product name

L-Plastin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LENAGCNKI GNFSTDIKDS KAYYHLLEQV APKGDEEGVP AVVIDMSGLR EKDDIQRAEC MLQQAERLGC RQFVTATDVV RGNPKLNLAF IANLFNRYPA LHKPENQDID WGALEGETRE ERTFRNWMNS LGVNPRVNHL YSDLSDALVI FQLYEKIKVP VDWNRVNKPP YPKLGGNMKK LENCNYAVEL GKNQAKFSLV GIGGQDLNEG NRTLTLALIW QLMRRYTLNI LEEIGGGQKV NDDIIVNWVN ETLREAEKSS SISSFKDPKI STSLPVLDLI DAIQPGSINY DLLKTENLND DEKLNNAKYA ISMARKIGAR VYALPEDLVE VNPKMVMTVF ACLMGKGMKR V (278-627 aa encoded by BC010271)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 13025-1-AP (L-Plastin antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 13025-1-AP (L-Plastin antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 13025-1-AP (L-Plastin antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Raji cells were stained with 0.2ug L-Plastin antibody (13025-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-L-Plastin (IP:13025-1-AP, 4ug; Detection:13025-1-AP 1:1000) with Jurkat cells lysate 2400ug.