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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-82000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SDVNSKQGMS RHPPAPDIPT FYPLSPGGVG QITPPLGWQG QPVYPITGGF RQPYPSSLSV DTSMSRFSHH MIPGPPGPHT TGIPHPAIVT PQVKQEHPHT DSDLMHVKPQ HEQRKEQEPK RPHIKKPLNA FMLY
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Changes in the Expression of Smooth Muscle Cell-Related Genes in Human Dermal Sheath Cup Cells Associated with the Treatment Outcome of Autologous Cell-Based Therapy for Male and Female Pattern Hair Loss.
Yoshida Y, Takahashi M, Yamanishi H, Nakazawa Y, Kishimoto J, Ohyama M
International journal of molecular sciences 2022 Jun 27;23(13)
International journal of molecular sciences 2022 Jun 27;23(13)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 by a LEF1 polyclonal antibody (Product # PA5-82000). Analysis in human cell lines SK-MEL-30 and A-549.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LEF1 in U-251 MG cells using a LEF1 polyclonal antibody (Product # PA5-82000). The analysis shows localization to nucleoplasm & cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LEF1 in human tonsil and pancreas tissues using a LEF1 polyclonal antibody (Product # PA5-82000). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LEF1 in human tonsil using a LEF1 polyclonal antibody (Product # PA5-82000). The analysis shows moderate nuclear positivity in non - germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LEF1 in human pancreas using a LEF1 polyclonal antibody (Product # PA5-82000). The analysis shows no nuclear positivity as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LEF1 in human lymph node using a LEF1 polyclonal antibody (Product # PA5-82000). The analysis shows moderate to strong nuclear positivity in non - germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LEF1 in human oral mucosa using a LEF1 polyclonal antibody (Product # PA5-82000). The analysis shows moderate nuclear positivity in subset of squamous epithelial cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effect of DPAC medium and CHIR99021 treatment on DSC cells. ( A , B ) Correlation between the treatment outcome score at 3, 6, 9, and 12 months vs. day 0 (3 M/0 M, 6 M/0 M, 9 M/0 M, and 12 M/0 M) or vs. placebo (3 M/p, 6 M/p, 9 M/p, and 12 M/p) during the clinical study using DSC cell lines [], and the expression levels of DP markers. Each value represents the correlation coefficient. Red: positive correlation; blue: inverse correlation. ( C ) Relative expression levels of Sox2, LEF1, Noggin, and BMP4 in three DSC cell lines (DSC#1-#3) cultured in medium (control) or with 3 uM CHIR99021 ( n = 3). Expression levels were normalized to GAPDH and the results are expressed as the mean +- S.D. Student's t -test, * p < 0.05, ** p < 0.01, *** p < 0.001. ( D ) Immunostaining of DSC cells cultured in control or CHIR99021-containing medium for SOX2 (red), LEF1 (green), and beta-catenin (red). Blue indicates Hoechst 33,342 nuclear staining. Arrowheads: beta-catenin nuclear translocation. Bars = 50 um.
