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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB1865 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1865, RRID:AB_1580923
- Product name
- SOX9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SOX9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression and biological role of the prostaglandin D synthase/SOX9 pathway in human ovarian cancer cells.
SOX9 is a key player in ultraviolet B-induced melanocyte differentiation and pigmentation.
Malki S, Bibeau F, Notarnicola C, Roques S, Berta P, Poulat F, Boizet-Bonhoure B
Cancer letters 2007 Oct 8;255(2):182-93
Cancer letters 2007 Oct 8;255(2):182-93
SOX9 is a key player in ultraviolet B-induced melanocyte differentiation and pigmentation.
Passeron T, Valencia JC, Bertolotto C, Hoashi T, Le Pape E, Takahashi K, Ballotti R, Hearing VJ
Proceedings of the National Academy of Sciences of the United States of America 2007 Aug 28;104(35):13984-9
Proceedings of the National Academy of Sciences of the United States of America 2007 Aug 28;104(35):13984-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SOX9 polyclonal antibody (Cat # PAB1865) in K-562 cell line lysates (35 ug/lane). SOX9 (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SOX9 (arrow) using SOX9 polyclonal antibody (Cat # PAB1865). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOX9 polyclonal antibody (Cat # PAB1865) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L) . Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with SOX9 polyclonal antibody (Cat # PAB1865) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
