M8421

antibody from MilliporeSigma / Merck KGaA

Targeting: MYH7 CMD1S, CMH1, MPD1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Protein array (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

M8421

Provider

MilliporeSigma / Merck KGaA

Product name

Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Feline, Goat, Guinea Pig, Hamster, Porcine, Rabbit, Sheep

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

NOQ7.5.4D

Storage

Store at -20°C

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 71

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.