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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB90961-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MYH7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human MYH7 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P12883- Isotype
- IgG
- Antibody clone number
- 2021A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease.
Zhao Y, Wang C, Wang C, Hong X, Miao J, Liao Y, Zhou L, Liu Y
Scientific reports 2018 Jun 12;8(1):8996
Scientific reports 2018 Jun 12;8(1):8996
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MYH7 by Western Blot. Western blot shows lysates of human heart (ventricle) tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MYH7 in Human Cardiomyocytes. MYH7 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
