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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-30086 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Proprotein Convertase 2/PCSK2 Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Proprotein Convertase 2/PCSK2 Antibody [NBP2-30086] - Western blot analysis of PCSK2 Antibody (N-term) (NBP2-30086) in mouse cerebellum tissue lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Proprotein Convertase 2/PCSK2 Antibody [NBP2-30086] - Confocal immunofluorescent analysis of PCSK2 Antibody (N-term)(NBP2-30086) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Proprotein Convertase 2/PCSK2 Antibody [NBP2-30086] - Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Proprotein Convertase 2/PCSK2 Antibody [NBP2-30086] - Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
