GTX23533

antibody from GeneTex

Targeting: PCSK2 NEC2, PC2, SPC2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX23533

Provider

GeneTex

Proper citation

GeneTex Cat#GTX23533, RRID:AB_385097

Product name

PC2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

PTBP1 is required for glucose-stimulated cap-independent translation of insulin granule proteins and Coxsackieviruses in beta cells.

Knoch KP, Nath-Sain S, Petzold A, Schneider H, Beck M, Wegbrod C, Sönmez A, Münster C, Friedrich A, Roivainen M, Solimena M
Molecular metabolism 2014 Aug;3(5):518-30

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Wolfram syndrome 1 gene (WFS1) product localizes to secretory granules and determines granule acidification in pancreatic beta-cells.

Hatanaka M, Tanabe K, Yanai A, Ohta Y, Kondo M, Akiyama M, Shinoda K, Oka Y, Tanizawa Y
Human molecular genetics 2011 Apr 1;20(7):1274-84

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of PC2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PC2 polyclonal antibody (GTX23533) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ?C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of PC2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PC2 polyclonal antibody (GTX23533) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ?C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.