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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB3857 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3857, RRID:AB_1575596
- Product name
- NEUROD1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NEUROD1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references NeuroD1/E47 regulates the E-box element of a novel zinc finger transcription factor, IA-1, in developing nervous system.
The Ala45Thr polymorphism of BETA2/NeuroD1 gene and susceptibility to type 2 diabetes mellitus in a Polish population.
NEUROD polymorphism Ala45Thr is associated with Type 1 diabetes mellitus in Czech children.
Breslin MB, Zhu M, Lan MS
The Journal of biological chemistry 2003 Oct 3;278(40):38991-7
The Journal of biological chemistry 2003 Oct 3;278(40):38991-7
The Ala45Thr polymorphism of BETA2/NeuroD1 gene and susceptibility to type 2 diabetes mellitus in a Polish population.
Malecki MT, Cyganek K, Klupa T, Sieradzki J
Acta diabetologica 2003 Jun;40(2):109-11
Acta diabetologica 2003 Jun;40(2):109-11
NEUROD polymorphism Ala45Thr is associated with Type 1 diabetes mellitus in Czech children.
Cinek O, Drevínek P, Sumník Z, Bendlová B, Sedláková P, Kolousková S, Snajderová M, Vavrinec J
Diabetes research and clinical practice 2003 Apr;60(1):49-56
Diabetes research and clinical practice 2003 Apr;60(1):49-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HepG2 cell lysate (35 ug/lane) with NEUROD1 polyclonal antibody (Cat # PAB3857).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ES cells were transiently transfected with flag-tagged mouse NEUROD1.Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NEUROD1 polyclonal antibody (Cat # PAB3857) at 1:100. NEUROD1 polyclonal antibody (Cat # PAB3857) showed strong and clear staining with similar pattern to the flag staining.(Supplied by Sally Lowell, Edinburgh University)
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEUROD1 polyclonal antibody (Cat # PAB3857) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
