GTX23447

antibody from GeneTex

Targeting: NFATC4 NFAT3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX23447

Provider

GeneTex

Proper citation

GeneTex Cat#GTX23447, RRID:AB_385053

Product name

NFATC4 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of NFAT3 in 25ug of various whole cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with NFAT3 antibody at a dilution of 1:1000 overnight at 4¢XC on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a proper secondary. Membranes were washed and chemiluminescent detection performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of NFAT3 in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with NFAT3 antibody at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a proper secondary antibody . Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of NFAT3 from MCF7 cell lysate. The antigen-antibody complex was formed by incubating 500£gg whole cell lysate with 3£gg of NFAT3 antibody overnight on a rocking platform at 4¢XC. The immune-complex was captured on 50£gl Protein A/G Agarose. Captured immune-complexes were washed and eluted. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were washed in TBS-0.1%Tween 20 and probed with NFAT3 antibody and a proper secondary antibody. Membranes were washed and chemiluminescent detection performed.