H00004776-D01P

antibody from Abnova Corporation

Targeting: NFATC4 NFAT3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004776-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004776-D01P, RRID:AB_1676995

Product name

NFATC4 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human NFATC4 protein.

Antigen sequence

MGAASCEDEELEFKLVFGEEKEAPPLGAGGLGEEL
DSEDAPPCCRLALGEPPPYGAAPIGIPRPPPPRPG
MHSPPPRPAPSPGTWESQPARSVRLGGPGGGAGGA
GGGRVLECPSIRITSISPTPEPPAALEDNPDAWGD
GSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSS
LSSWSFFSDASDEAALYAACDEVESELNEAASRFG
LGSPLPSPRASPRPWTPEDPWSLYGPSPGGRGPED
SWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSA
SPALSRRGSLGEEGSEPPPPPPLPLARDPGSPGPF
DYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASC
NGKLPLGAEESVAPPGGSRKEVAGMDYLAVPSPLA
WSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELR
IEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLL
GYSEKPLTLQMFIGTADERNLRPHAFYQVHRITGK
MVATASYEAVVSGTKVLEMTLLPENNMAANIDCAG
ILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQ
GGGKVVSVQAASVPIECSQRSAQELPQVEAYSPSA
CSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQW
EEEATVNRLQSNEVTLTLTVPEYSNKRVSRPVQVY
FYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRG
FPSASATPFGTDMDFSPPRPPYPSYPHEDPACETP
YLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRG
TTGCAQPPAVSFLPRPFPSDPYGGRGSSFSLGLPF
SPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGY
NKVGPGYGPGEGAPEQEKSRGGYSSGFRDSVPIQG
ITLEEVSEIIGRDLSGFPAPPGEEPPA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NFATC4 expression in transfected 293T cell line (H00004776-T02) by NFATC4 MaxPab polyclonal antibody.Lane 1: NFATC4 transfected lysate(95.40 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to NFATC4 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between NFATC4 and MAPK14. HeLa cells were stained with anti-NFATC4 rabbit purified polyclonal 1:1200 and anti-MAPK14 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)