Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- STJ119216 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-T antibody (226-435) (STJ119216)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-T (226-435) is suitable for use in Western Blot and Immunofluorescence.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store in a freezer at-20°C and avoid freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NCI-H460 cells using Brachyury rabbit polyclonal antibody (STJ119216) aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- WesBrachyuryern bloBrachyury analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury rabbit polyclonal antibody (STJ119216) aBrachyury 1:1000 diluBrachyuryion. Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG (H+L) aBrachyury 1:10000 diluBrachyuryion. LysaBrachyuryes/proBrachyuryeins: 25ug per lane. Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury. DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury. Exposure Brachyuryime: 30s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA