A06521

antibody from Boster Biological Technology

Targeting: WRNIP1 bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

A06521

Provider

Boster Biological Technology

Product name

Anti-WHIP WRNIP1 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for WRNIP1 detection. Tested positive for ELISA,Immunohistochemistry,Western Blot, in human, mouse, rat, -

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of WRNIP1 using anti-WRNIP1 antibody (A06521).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRNIP1 antigen affinity purified polyclonal antibody (Catalog # A06521) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WRNIP1.

Additional image