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LearnPAB10242
antibody from Abnova Corporation
Targeting: WRNIP1
bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- PAB10242 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10242, RRID:AB_1717136
- Product name
- WRNIP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of WRNIP1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A novel protein interacts with the Werner's syndrome gene product physically and functionally.
Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T
The Journal of biological chemistry 2001 Jun 8;276(23):20364-9
The Journal of biological chemistry 2001 Jun 8;276(23):20364-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Fig. 2Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) with and without pre-incubation with blocking peptide.Testing was performed on antiserum prior to affinity purification.Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WRNIP1.Approximately 30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE.Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 KDa is detected.The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Fig. 1Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) to detect human WRNIP1 present in a HEK293 whole cell lysate.Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE.Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 KDa is detected.The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 protein.See Figure 2 for the results of peptide competition experiments.The blot was incubated with a 1 : 200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.
