600-401-676
antibody from Rockland Immunochemicals, Inc.
Targeting: WRNIP1
bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-676 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-676, RRID:AB_2288536
- Product name
- Anti-Human WHIP (RABBIT) Antibody - 600-401-676
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis is shown using Rockland's Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot