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LearnNB110-61626
antibody from Novus Biologicals
Targeting: WRNIP1
bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB110-61626 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-61626, RRID:AB_2216082
- Product name
- Rabbit Polyclonal WHIP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WHIP Antibody [NB110-61626] - Detection of Human and Mouse WRNIP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-WRNIP1 antibody NB110-61626 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WHIP Antibody [NB110-61626] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-WRNIP1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: WHIP Antibody [NB110-61626] - Detection of human WRNIP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRNIP1 antibody NB110-61626 used for IP at 6 ug/mg lysate. WRNIP1 was also immunoprecipitated by another rabbit anti-WRNIP1 antibody. For blotting immunoprecipitated WRNIP1, NB110-61626 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WHIP Antibody [NB110-61626] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-WRNIP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
