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- References [11]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-24710 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Smad7 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.
- Reactivity
- Human, Mouse, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references MicroRNA profiles in plasma samples from young metabolically healthy obese patients and miRNA-21 are associated with diastolic dysfunction via TGF-β1/Smad pathway.
Alternative Splice Forms of CYLD Mediate Ubiquitination of SMAD7 to Prevent TGFB Signaling and Promote Colitis.
A critical role for the mTORC2 pathway in lung fibrosis.
TGF-β induced TMEPAI/PMEPA1 inhibits canonical Smad signaling through R-Smad sequestration and promotes non-canonical PI3K/Akt signaling by reducing PTEN in triple negative breast cancer.
Retinoic acid induced repair in the lung of adult hyperoxic mice, reducing transforming growth factor-β1 (TGF-β1) mediated abnormal alterations.
Transcriptional induction of salt-inducible kinase 1 by transforming growth factor β leads to negative regulation of type I receptor signaling in cooperation with the Smurf2 ubiquitin ligase.
Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family.
MicroRNA-377 is up-regulated and can lead to increased fibronectin production in diabetic nephropathy.
Small bowel carcinoid (enterochromaffin cell) neoplasia exhibits transforming growth factor-beta1-mediated regulatory abnormalities including up-regulation of C-Myc and MTA1.
Inhibition of Smad7, a negative regulator of TGF-beta signaling, suppresses autoimmune encephalomyelitis.
Modulation of TGF-beta signaling by proinflammatory cytokines in articular chondrocytes.
Yang P, Dong X, Zhang Y
Journal of clinical laboratory analysis 2020 Jun;34(6):e23246
Journal of clinical laboratory analysis 2020 Jun;34(6):e23246
Alternative Splice Forms of CYLD Mediate Ubiquitination of SMAD7 to Prevent TGFB Signaling and Promote Colitis.
Tang Y, Reissig S, Glasmacher E, Regen T, Wanke F, Nikolaev A, Gerlach K, Popp V, Karram K, Fantini MC, Schattenberg JM, Galle PR, Neurath MF, Weigmann B, Kurschus FC, Hövelmeyer N, Waisman A
Gastroenterology 2019 Feb;156(3):692-707.e7
Gastroenterology 2019 Feb;156(3):692-707.e7
A critical role for the mTORC2 pathway in lung fibrosis.
Chang W, Wei K, Ho L, Berry GJ, Jacobs SS, Chang CH, Rosen GD
PloS one 2014;9(8):e106155
PloS one 2014;9(8):e106155
TGF-β induced TMEPAI/PMEPA1 inhibits canonical Smad signaling through R-Smad sequestration and promotes non-canonical PI3K/Akt signaling by reducing PTEN in triple negative breast cancer.
Singha PK, Pandeswara S, Geng H, Lan R, Venkatachalam MA, Saikumar P
Genes & cancer 2014 Sep;5(9-10):320-36
Genes & cancer 2014 Sep;5(9-10):320-36
Retinoic acid induced repair in the lung of adult hyperoxic mice, reducing transforming growth factor-β1 (TGF-β1) mediated abnormal alterations.
Kayalar O, Oztay F
Acta histochemica 2014 Jun;116(5):810-9
Acta histochemica 2014 Jun;116(5):810-9
Transcriptional induction of salt-inducible kinase 1 by transforming growth factor β leads to negative regulation of type I receptor signaling in cooperation with the Smurf2 ubiquitin ligase.
Lönn P, Vanlandewijck M, Raja E, Kowanetz M, Watanabe Y, Kowanetz K, Vasilaki E, Heldin CH, Moustakas A
The Journal of biological chemistry 2012 Apr 13;287(16):12867-78
The Journal of biological chemistry 2012 Apr 13;287(16):12867-78
Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family.
Hogg K, Etherington SL, Young JM, McNeilly AS, Duncan WC
Endocrinology 2010 Mar;151(3):1247-56
Endocrinology 2010 Mar;151(3):1247-56
MicroRNA-377 is up-regulated and can lead to increased fibronectin production in diabetic nephropathy.
Wang Q, Wang Y, Minto AW, Wang J, Shi Q, Li X, Quigg RJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Dec;22(12):4126-35
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Dec;22(12):4126-35
Small bowel carcinoid (enterochromaffin cell) neoplasia exhibits transforming growth factor-beta1-mediated regulatory abnormalities including up-regulation of C-Myc and MTA1.
Kidd M, Modlin IM, Pfragner R, Eick GN, Champaneria MC, Chan AK, Camp RL, Mane SM
Cancer 2007 Jun 15;109(12):2420-31
Cancer 2007 Jun 15;109(12):2420-31
Inhibition of Smad7, a negative regulator of TGF-beta signaling, suppresses autoimmune encephalomyelitis.
Kleiter I, Pedré X, Mueller AM, Poeschl P, Couillard-Despres S, Spruss T, Bogdahn U, Giegerich G, Steinbrecher A
Journal of neuroimmunology 2007 Jul;187(1-2):61-73
Journal of neuroimmunology 2007 Jul;187(1-2):61-73
Modulation of TGF-beta signaling by proinflammatory cytokines in articular chondrocytes.
Roman-Blas JA, Stokes DG, Jimenez SA
Osteoarthritis and cartilage 2007 Dec;15(12):1367-77
Osteoarthritis and cartilage 2007 Dec;15(12):1367-77
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.
