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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324865 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Smad7 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Smad7 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SMAD7
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Smad7 Antibody (Center) (Cat. #TA324865) in ZR-75-1 cell line lysates (35ug/lane). Smad7 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Fluorescent confocal image of A431 cell stained with Smad7 Antibody (Center)(Cat#TA324865). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25, 1 h at 37??). For secondary antibody, Alexa Fluor?? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37??). Nuclei were counterstained with DAPI (blue) (10 ?ug/ml, 10 min).Smad7 immunoreactivity is localized to cytoplasm significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Smad7 Antibody (Center) (Cat. #TA324865) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Smad7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
