LS-C185493

antibody from LSBio

Targeting: ZNF143 pHZ-1, SBF, STAF

Western blot (Enhanced validation)

Immunocytochemistry (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185493

Provider

LSBio

Product name

ZNF143 / STAF Antibody (aa409-621) LS-C185493

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.