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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324734 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal RUNX1 Antibody (S276)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal RUNX1 Antibody (S276)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RUNX1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- RUNX1 Antibody (S276) (Cat. #TA324734) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the RUNX1 antibody detected the RUNX1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of RUNX1 Antibody (S276)(Cat#TA324734) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) (Cat.#TA324734), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- RUNX1 Antibody (S276) (Cat. #TA324734) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
