Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C150101 - Provider product page
- Provider
- LSBio
- Product name
- Anti-Vimentin Antibody (aa2-466) LS-C150101
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 25µg
- Storage
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Vimentin in A549 Human Cell Line. Vimentin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/ml TGF-beta for 48 hours using Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 ug/ml Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).