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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- LS-C180202 - Provider product page
- Provider
- LSBio
- Product name
- Vimentin Antibody (clone 2D1) LS-C180202
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D1
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Western Blot: Vimentin Antibody (2D1) - Western blot of crude extract HeLa. Lane 18 was probed with Vimentin antibody. Note the strong clean band at the expected molecular weight of 50kDa. Lane 17 was probed with a monoclonal antibody to Vimentin. Lane 15 was probed with an antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and Lane 16 with an antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.
