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Western blot
Immunocytochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- LS-C180203 - Provider product page
- Provider
- LSBio
- Product name
- Vimentin Antibody (clone 2A52) LS-C180203
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A52
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- Western Blot: Vimentin Antibody (2A52) - Note the strong clean band at the expected molecular weight of 50kDa. Lane 17 was probed with another monoclonal antibody to Vimentin. Lane 15 was probed with an antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and Lane 16 with an antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.
