GTX629743

antibody from GeneTex

Targeting: VIM

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629743

Provider

GeneTex

Product name

Vimentin antibody [GT7812]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

References

Submitted references

Epiproteome profiling of cadmium-transformed human bronchial epithelial cells by quantitative histone post-translational modification-enzyme-linked immunosorbent assay.

Liang ZL, Wu DD, Yao Y, Yu FY, Yang L, Tan HW, Hylkema MN, Rots MG, Xu YM, Lau ATY
Journal of applied toxicology : JAT 2018 Jun;38(6):888-895

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SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway.

Li SW, Wang CY, Jou YJ, Yang TC, Huang SH, Wan L, Lin YJ, Lin CW
Scientific reports 2016 May 13;6:25754

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Adipose-derived stem cells from the breast.

Yang J, Xiong L, Wang R, Sun J, Hirche C
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences 2014 Feb;19(2):112-6

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

Antibody Lot Number

41983

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extract10% SDS-PAGEVimentin antibody [GT7812] (GTX629743) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein by western blot analysis.A. 30 ?g C8D30 whole cell lysate/extractB. 30 ?g NIH-3T3 whole cell lysate/extractC. 30 ?g C2C12 whole cell lysate/extract10% SDS-PAGEVimentin antibody [GT7812] (GTX629743) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein by Western blot analysis.A. 30 ?g Rat-2 whole cell lysate/extract10 % SDS-PAGEVimentin antibody [GT7812] (GTX629743) dilution: 1:10000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.Green: Vimentin protein stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000. Blue: DAPI.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm in human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.