LS-B1870

antibody from LSBio

Targeting: VIM

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B1870

Provider

LSBio

Product name

IHC-plus™ Vimentin Antibody (clone VI-10) LS-B1870

Antibody type

Monoclonal

Description

Purified by precipitation and chromatography. Greater than 95% by SDS-PAGE

Reactivity

Human, Mouse, Rat, Chicken/Avian, Porcine

Host

Mouse

Isotype

IgM

Antibody clone number

VI-10

Storage

Store at 2°C to 8°C. Do not freeze.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-Vimentin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.