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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- OAPC00084 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- Anti-Vimentin
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Chicken/Avian
- Vial size
- 100µl
- Storage
- Store at -20°C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (catalog #620-GFAP) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.
