Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C203044 - Provider product page
- Provider
- LSBio
- Product name
- Vimentin Antibody LS-C203044
- Antibody type
- Monoclonal
- Description
- Immunoaffinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Long term: aliquot and store at -20°C. Reconstituted: store at 2°C to 8°C for up to 3 weeks. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysates using 1 ug/ml Rabbit Anti-Vimentin Polyclonal Antibody. Lane 1, 3: HeLa cell lysate. Lane 2, 4: HEK293 cell lysate. Primary antibody: Lane 1, 2: Rabbit Anti-Vimentin Polyclonal Antibody. Lane 3, 4: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size 53 kD. Observed band size 53 kD. Additional band size 30 kD (Possible vimentin fragment).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot of cell lysates using 1 ug/ml Rabbit Anti-Vimentin Polyclonal Antibody. Lane 1, 3: HeLa cell lysate. Lane 2, 4: HEK293 cell lysate. Primary antibody: Lane 1, 2: Rabbit Anti-Vimentin Polyclonal Antibody. Lane 3, 4: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size 53 kD. Observed band size 53 kD. Additional band size 30 kD (Possible vimentin fragment).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot of cell lysates using 1 ug/ml Rabbit Anti-Vimentin Polyclonal Antibody. Lane 1, 3: HeLa cell lysate. Lane 2, 4: HEK293 cell lysate. Primary antibody: Lane 1, 2: Rabbit Anti-Vimentin Polyclonal Antibody. Lane 3, 4: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size 53 kD. Observed band size 53 kD. Additional band size 30 kD (Possible vimentin fragment).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of human spleen tissue slide (Paraffin embedded) using Rabbit Anti-Vimentin Polyclonal Antibody (Left) and Purified Rabbit IgG (Whole molecule) Control (Right).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of human spleen tissue slide (Paraffin embedded) using Rabbit Anti-Vimentin Polyclonal Antibody (Left) and Purified Rabbit IgG (Whole molecule) Control (Right).