LS-C98614

antibody from LSBio

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C98614

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C98614, RRID:AB_2216398

Product name

Vimentin Antibody (aa152-181) LS-C98614

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of Vimentin Antibody in A2058,A375 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified antibody.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of Vimentin (arrow) using Vimentin Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate tissue followed by peroxidase-conjugated secondary antibody and DAB staining.