- GTX112661 - GeneTex VIM antibody

Submitted references Upregulation of LGALS1 is associated with oral cancer metastasis.

Li JM, Tseng CW, Lin CC, Law CH, Chien YA, Kuo WH, Chou HC, Wang WC, Chan HL
Therapeutic advances in medical oncology 2018;10:1758835918794622

Activation of fibroblasts by nicotine promotes the epithelial-mesenchymal transition and motility of breast cancer cells.

Chen PC, Lee WY, Ling HH, Cheng CH, Chen KC, Lin CW
Journal of cellular physiology 2018 Jun;233(6):4972-4980

Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.

Chen CY, Chen CC, Shieh TM, Hsueh C, Wang SH, Leu YL, Lian JH, Wang TH
International journal of molecular sciences 2018 Jan 27;19(2)

Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.

Terasaki M, Ogawa Y, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Anticancer research 2018 Apr;38(4):2169-2179

Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer.

Lee WY, Kuo CC, Lin BX, Cheng CH, Chen KC, Lin CW
International journal of molecular sciences 2017 Sep 23;18(10)

Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.

Li CH, Liu CW, Tsai CH, Peng YJ, Yang YH, Liao PL, Lee CC, Cheng YW, Kang JJ
Archives of toxicology 2017 May;91(5):2165-2178

Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.

Fujiki K, Inamura H, Miyayama T, Matsuoka M
The Journal of biological chemistry 2017 May 12;292(19):7942-7953

Metformin inhibits castration-induced EMT in prostate cancer by repressing COX2/PGE2/STAT3 axis.

Tong D, Liu Q, Liu G, Xu J, Lan W, Jiang Y, Xiao H, Zhang D, Jiang J
Cancer letters 2017 Mar 28;389:23-32

Nm23-H1-stabilized hnRNPA2/B1 promotes internal ribosomal entry site (IRES)-mediated translation of Sp1 in the lung cancer progression.

Hung CY, Wang YC, Chuang JY, Young MJ, Liaw H, Chang WC, Hung JJ
Scientific reports 2017 Aug 22;7(1):9166

Co-Orientation: Quantifying Simultaneous Co-Localization and Orientational Alignment of Filaments in Light Microscopy.

Nieuwenhuizen RP, Nahidiazar L, Manders EM, Jalink K, Stallinga S, Rieger B
PloS one 2015;10(7):e0131756

Investigation of multipotent postnatal stem cells from human maxillary sinus membrane.

Guo J, Weng J, Rong Q, Zhang X, Zhu S, Huang D, Li X, Chen S
Scientific reports 2015 Jun 29;5:11660

Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.

Zhao B, Xu B, Hu W, Song C, Wang F, Liu Z, Ye M, Zou H, Miao QR
Journal of proteomics 2015 Jan 1;112:38-52

Neuregulin-1-mediated ErbB2-ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation.

Fock V, Plessl K, Draxler P, Otti GR, Fiala C, Knöfler M, Pollheimer J
Journal of cell science 2015 Dec 1;128(23):4306-16

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 40093

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX112661 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects VIM protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g NIH-3T3 whole cell lysate/extract10 % SDS-PAGEVimentin antibody (GTX112661) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. Vimentin antibody (GTX112661) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Vimentin antibody (GTX112661) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects Vimentin protein at cytosol on mouse lymph node by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. Vimentin antibody (GTX112661) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin antibody (GTX112661) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Vimentin antibody (GTX112661) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody (GTX112661) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (GTX112661) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

GTX112661

Antibody data