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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX80814 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80814, RRID:AB_625559
- Product name
- Vimentin antibody [J144]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of vimentin in rat brain.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of vimentin in rt brain cells using GTX80814.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Vimentin in HEK293 cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Vimentin antibody [J144] at a dilution of 1:100-1:200 over night at 4 ¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
