Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM32993PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Vimentin
- Antibody type
- Monoclonal
- Antigen
- Recombinant human vimentin purified from E. coli.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D1
- Vial size
- 0.1 ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot of HeLa cells showing specific immunolabeling of the ~50k Vimentin protein.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mixed neuron/glial cultures stained with anti-Vimentin (green) and Rabbit anti-GFAP antibody (Cat.-No AP08673SU-N) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.
