Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX132608 - Provider product page
- Provider
- GeneTex
- Product name
- Vimentin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX132608) diluted at 1:4000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX132608) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX132608) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX132608) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX132608) diluted at 1:4000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin protein stained by Vimentin antibody (GTX132608) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.