GTX27752

antibody from GeneTex

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX27752

Provider

GeneTex

Proper citation

GeneTex Cat#GTX27752, RRID:AB_373289

Product name

Vimentin antibody [VI-01]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Porcine

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody GTX27752. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (GTX27752) Lane 3,4: immunostaining with anti-human Vimentin (GTX23974)

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (GTX27752): Dyomics 547. Nuclei are stained with DAPI (blue)

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (GTX27752): Dyomics 547. Nuclei are stained with DAPI (blue)