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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2587 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2587, RRID:AB_1581857
- Product name
- VIM polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of VIM.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Interaction of surfactant protein A with the intermediate filaments desmin and vimentin.
Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells.
Filensin: a new vimentin-binding, polymerization-competent, and membrane-associated protein of the lens fiber cell.
Garcia-Verdugo I, Synguelakis M, Degrouard J, Franco CA, Valot B, Zivy M, Chaby R, Tanfin Z
Biochemistry 2008 May 6;47(18):5127-38
Biochemistry 2008 May 6;47(18):5127-38
Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells.
Whipple RA, Balzer EM, Cho EH, Matrone MA, Yoon JR, Martin SS
Cancer research 2008 Jul 15;68(14):5678-88
Cancer research 2008 Jul 15;68(14):5678-88
Filensin: a new vimentin-binding, polymerization-competent, and membrane-associated protein of the lens fiber cell.
Merdes A, Brunkener M, Horstmann H, Georgatos SD
The Journal of cell biology 1991 Oct;115(2):397-410
The Journal of cell biology 1991 Oct;115(2):397-410
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of VIM (arrow) using rabbit VIM polyclonal antibody (Cat # PAB2587). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinomareacted with VIM polyclonal antibody (Cat # PAB2587), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
