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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA1102 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Vimentin Antibody (monoclonal)
- Antibody type
- Monoclonal
- Antigen
- Pig eye lens vimentin.
- Description
- Ascites
- Reactivity
- Human, Rat, Porcine, Rabbit
- Vial size
- 100μg/vial
- Concentration
- Lyophilized
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100μg/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Vimentin using anti- Vimentin antibody (MA1102). Vimentin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Vimentin Antibody (MA1102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
