NB300-223

antibody from Novus Biologicals

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-223

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-223, RRID:AB_10003206

Product name

Chicken Polyclonal Vimentin Antibody

Antibody type

Polyclonal

Description

IgY purified.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine

Host

Chicken/Avian

Isotype

IgY

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Cooperative roles of PAK1 and filamin A in regulation of vimentin assembly and cell extension formation.

Ding I, Ostrowska-Podhorodecka Z, Lee W, Liu RSC, Carneiro K, Janmey PA, McCulloch CA
Biochimica et biophysica acta. Molecular cell research 2020 Sep;1867(9):118739

---

Juvenile mild traumatic brain injury elicits distinct spatiotemporal astrocyte responses.

Clément T, Lee JB, Ichkova A, Rodriguez-Grande B, Fournier ML, Aussudre J, Ogier M, Haddad E, Canini F, Koehl M, Abrous DN, Obenaus A, Badaut J
Glia 2020 Mar;68(3):528-542

---

Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2.

Zhou X, Wahane S, Friedl MS, Kluge M, Friedel CC, Avrampou K, Zachariou V, Guo L, Zhang B, He X, Friedel RH, Zou H
Nature neuroscience 2020 Mar;23(3):337-350

---

3-D culture and endothelial cells improve maturity of human pluripotent stem cell-derived hepatocytes.

Ardalani H, Sengupta S, Harms V, Vickerman V, Thomson JA, Murphy WL
Acta biomaterialia 2019 Sep 1;95:371-381

---

Distinctive requirement of PKCε in the control of Rho GTPases in epithelial and mesenchymally transformed lung cancer cells.

Casado-Medrano V, Barrio-Real L, Wang A, Cooke M, Lopez-Haber C, Kazanietz MG
Oncogene 2019 Jul;38(27):5396-5412

---

Loss of Vimentin Enhances Cell Motility through Small Confining Spaces.

Patteson AE, Pogoda K, Byfield FJ, Mandal K, Ostrowska-Podhorodecka Z, Charrier EE, Galie PA, Deptuła P, Bucki R, McCulloch CA, Janmey PA
Small (Weinheim an der Bergstrasse, Germany) 2019 Dec;15(50):e1903180

---

Macrophage Transitions in Heart Valve Development and Myxomatous Valve Disease.

Hulin A, Anstine LJ, Kim AJ, Potter SJ, DeFalco T, Lincoln J, Yutzey KE
Arteriosclerosis, thrombosis, and vascular biology 2018 Mar;38(3):636-644

---

Paracrine Fibroblast Growth Factor Initiates Oncogenic Synergy with Epithelial FGFR/Src Transformation in Prostate Tumor Progression.

Li Q, Ingram L, Kim S, Beharry Z, Cooper JA, Cai H
Neoplasia (New York, N.Y.) 2018 Mar;20(3):233-243

---

Magnetically Guided Self-Assembly and Coding of 3D Living Architectures.

Tocchio A, Durmus NG, Sridhar K, Mani V, Coskun B, El Assal R, Demirci U
Advanced materials (Deerfield Beach, Fla.) 2018 Jan;30(4)

---

Nano-sized Al₂O₃ particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts.

Li, Wang C, Li Z, Wang H, He J, Zhu J, Zhang Y, Shen C, Xiao F, Gao Y, Zhang X, Li Y, Wang P, Peng J, Cai G, Zuo B, Yang Y, Shen Y, Song W, Zhang X, Shen L, Chen X
Cell death & disease 2018 Aug 6;9(8):840

---

PyMT-1099, a versatile murine cell model for EMT in breast cancer.

Saxena M, Kalathur RKR, Neutzner M, Christofori G
Scientific reports 2018 Aug 14;8(1):12123

---

Recipient Glycemic Micro-environments Govern Therapeutic Effects of Mesenchymal Stem Cell Infusion on Osteopenia.

Sui BD, Hu CH, Zheng CX, Shuai Y, He XN, Gao PP, Zhao P, Li M, Zhang XY, He T, Xuan K, Jin Y
Theranostics 2017;7(5):1225-1244

---

Contribution of Extra-Cardiac Cells in Murine Heart Valves is Age-Dependent.

Anstine LJ, Horne TE, Horwitz EM, Lincoln J
Journal of the American Heart Association 2017 Oct 20;6(10)

---

Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.

Mao L, Gao W, Chen S, Song Y, Song C, Zhou Z, Zhao H, Zhou K, Wang W, Zhu K, Liu C, Mei X
Cell death & disease 2017 Nov 2;8(11):e3162

---

Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice.

Nakamura R, Koshiba-Takeuchi K, Tsuchiya M, Kojima M, Miyazawa A, Ito K, Ogawa H, Takeuchi JK
Development, growth & differentiation 2016 May;58(4):367-82

---

Increased collagen cross-linking is a signature of dystrophin-deficient muscle.

Smith LR, Hammers DW, Sweeney HL, Barton ER
Muscle & nerve 2016 Jun;54(1):71-8

---

Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow.

Calibasi Kocal G, Güven S, Foygel K, Goldman A, Chen P, Sengupta S, Paulmurugan R, Baskin Y, Demirci U
Scientific reports 2016 Dec 2;6:38221

---

Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments.

Behrens J, Solga R, Ziemann A, Rastetter RH, Berwanger C, Herrmann H, Noegel AA, Clemen CS
European journal of cell biology 2016 Aug;95(8):239-51

---

Mitochondrial catalase suppresses naturally occurring lung cancer in old mice.

Ge X, Pettan-Brewer C, Morton J, Carter K, Fatemi S, Rabinovitch P, Ladiges WC
Pathobiology of aging & age related diseases 2015;5:28776

---

Attenuation of Cerebral Ischemic Injury in Smad1 Deficient Mice.

Wong JK, Chen L, Huang Y, Sehba FA, Friedel RH, Zou H
PloS one 2015;10(8):e0136967

---

Lgr6 marks nail stem cells and is required for digit tip regeneration.

Lehoczky JA, Tabin CJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Oct 27;112(43):13249-54

---

Glial and perivascular structures in the subfornical organ: distinguishing the shell and core.

Pócsai K, Kálmán M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2015 May;63(5):367-83

---

Up-regulation of neural and cell cycle-related microRNAs in brain of amyotrophic lateral sclerosis mice at late disease stage.

Marcuzzo S, Bonanno S, Kapetis D, Barzago C, Cavalcante P, D'Alessandro S, Mantegazza R, Bernasconi P
Molecular brain 2015 Jan 28;8:5

---

Enhancing repair of full-thickness excisional wounds in a murine model: Impact of tissue-engineered biological dressings featuring human differentiated adipocytes.

Morissette Martin P, Maux A, Laterreur V, Mayrand D, L Gagné V, Moulin VJ, Fradette J
Acta biomaterialia 2015 Aug;22:39-49

---

ARF inhibits the growth and malignant progression of non-small-cell lung carcinoma.

Busch SE, Moser RD, Gurley KE, Kelly-Spratt KS, Liggitt HD, Kemp CJ
Oncogene 2014 May 15;33(20):2665-73

---

Substrate stiffness regulates solubility of cellular vimentin.

Murray ME, Mendez MG, Janmey PA
Molecular biology of the cell 2014 Jan;25(1):87-94

---

Acute and chronic mu opioids differentially regulate thrombospondins 1 and 2 isoforms in astrocytes.

Phamduong E, Rathore MK, Crews NR, D'Angelo AS, Leinweber AL, Kappera P, Krenning TM, Rendell VR, Belcheva MM, Coscia CJ
ACS chemical neuroscience 2014 Feb 19;5(2):106-14

---

Dysregulation of PAD4-mediated citrullination of nuclear GSK3β activates TGF-β signaling and induces epithelial-to-mesenchymal transition in breast cancer cells.

Stadler SC, Vincent CT, Fedorov VD, Patsialou A, Cherrington BD, Wakshlag JJ, Mohanan S, Zee BM, Zhang X, Garcia BA, Condeelis JS, Brown AM, Coonrod SA, Allis CD
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 16;110(29):11851-6

---

Esophageal epithelial and mesenchymal cross-talk leads to features of epithelial to mesenchymal transition in vitro.

Muir AB, Lim DM, Benitez AJ, Modayur Chandramouleeswaran P, Lee AJ, Ruchelli ED, Spergel JM, Wang ML
Experimental cell research 2013 Apr 1;319(6):850-9

---

Genomic analysis of reactive astrogliosis.

Zamanian JL, Xu L, Foo LC, Nouri N, Zhou L, Giffard RG, Barres BA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 May 2;32(18):6391-410

---

Epidermal growth factor receptor and mutant p53 expand an esophageal cellular subpopulation capable of epithelial-to-mesenchymal transition through ZEB transcription factors.

Ohashi S, Natsuizaka M, Wong GS, Michaylira CZ, Grugan KD, Stairs DB, Kalabis J, Vega ME, Kalman RA, Nakagawa M, Klein-Szanto AJ, Herlyn M, Diehl JA, Rustgi AK, Nakagawa H
Cancer research 2010 May 15;70(10):4174-84

---

Met induces mammary tumors with diverse histologies and is associated with poor outcome and human basal breast cancer.

Ponzo MG, Lesurf R, Petkiewicz S, O'Malley FP, Pinnaduwage D, Andrulis IL, Bull SB, Chughtai N, Zuo D, Souleimanova M, Germain D, Omeroglu A, Cardiff RD, Hallett M, Park M
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 4;106(31):12903-8

---

Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation.

Johnstone CN, Mongroo PS, Rich AS, Schupp M, Bowser MJ, Delemos AS, Tobias JW, Liu Y, Hannigan GE, Rustgi AK
Molecular and cellular biology 2008 Jan;28(2):687-704

---

The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation.

Okawa T, Michaylira CZ, Kalabis J, Stairs DB, Nakagawa H, Andl CD, Johnstone CN, Klein-Szanto AJ, El-Deiry WS, Cukierman E, Herlyn M, Rustgi AK
Genes & development 2007 Nov 1;21(21):2788-803

---

Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor.

Rankin EB, Tomaszewski JE, Haase VH
Cancer research 2006 Mar 1;66(5):2576-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Vimentin Antibody [NB300-223] - Attenuated reactive astrocytosis after stroke in Smad1 cKO mice. Enlarged IHC images of boxed areas at the cortical peri-infarct area with the indicated reactive astrocyte markers GFAP, Nestin, and Vimentin. Enlarged images of GFAP IHC highlight the hypertrophic morphology of GFAP+ astrocytes in mutants. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0136967), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - Infarcted mouse heart frozen sections stained with vimentin antibody at 1:200. Secondary antibody at 1:500. Fixation with 4% PFA. IHC-Fr image submitted by a verified customer review.