Explore
Validate
Learn
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-11711 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPSAB1 Monoclonal Antibody (AA1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-11711 targets Mast Cell Tryptase in IHC (P) and WB applications and shows reactivity with Canine, Feline, Human, and Non-human primate samples.
- Antibody clone number
- AA1
- Concentration
- 50 mg/mL
Submitted references Mast Cells Differentiated in Synovial Fluid and Resident in Osteophytes Exalt the Inflammatory Pathology of Osteoarthritis.
Airway epithelium-shifted mast cell infiltration regulates asthmatic inflammation via IL-33 signaling.
The Impact of Mast Cell Density on the Progression of Bone Disease in Multiple Myeloma Patients.
Methods for the study of mast cells in cancer.
The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production.
Colonic mucosal immune activity in irritable bowel syndrome: comparison with healthy controls and patients with ulcerative colitis.
Activation of cutaneous immune responses in complex regional pain syndrome.
Regional Differences in Chronic Stress-induced Alterations in Mast Cell and Protease-activated Receptor-2-positive Cell Numbers in the Colon of Ws/Ws Rats.
The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
Histamine induces the neuronal hypertrophy and increases the mast cell density in gastrointestinal tract.
Pressure overload leads to an increase of cardiac resident stem cells.
Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps.
Soluble mediators released from PI-IBS patients' colon induced alteration of mast cell: involvement of reactive oxygen species.
Identification of mast cells and mast cell subpopulations.
Immediate anaphylactic death following antibiotics injection: splenic eosinophilia easily revealed by pagoda red stain.
Postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents: evaluation of clinical laboratory parameters and immunohistochemistry in three cases.
Interstitial Cajal-like cells in rat mesentery: an ultrastructural and immunohistochemical approach.
Elastofibroma: a clinicopathologic and immunohistochemical study of seven cases and literature review.
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.
Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.
Kulkarni P, Harsulkar A, Märtson AG, Suutre S, Märtson A, Koks S
International journal of molecular sciences 2022 Jan 4;23(1)
International journal of molecular sciences 2022 Jan 4;23(1)
Airway epithelium-shifted mast cell infiltration regulates asthmatic inflammation via IL-33 signaling.
Altman MC, Lai Y, Nolin JD, Long S, Chen CC, Piliponsky AM, Altemeier WA, Larmore M, Frevert CW, Mulligan MS, Ziegler SF, Debley JS, Peters MC, Hallstrand TS
The Journal of clinical investigation 2019 Nov 1;129(11):4979-4991
The Journal of clinical investigation 2019 Nov 1;129(11):4979-4991
The Impact of Mast Cell Density on the Progression of Bone Disease in Multiple Myeloma Patients.
Vyzoukaki R, Tsirakis G, Pappa CA, Devetzoglou M, Tzardi M, Alexandrakis MG
International archives of allergy and immunology 2015;168(4):263-8
International archives of allergy and immunology 2015;168(4):263-8
Methods for the study of mast cells in cancer.
Blatner NR, Tsai F, Khazaie K
Methods in molecular biology (Clifton, N.J.) 2015;1220:443-60
Methods in molecular biology (Clifton, N.J.) 2015;1220:443-60
The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production.
Ningyan G, Xu Y, Hongfei S, Jingjing C, Min C
PloS one 2015;10(3):e0122482
PloS one 2015;10(3):e0122482
Colonic mucosal immune activity in irritable bowel syndrome: comparison with healthy controls and patients with ulcerative colitis.
Ahn JY, Lee KH, Choi CH, Kim JW, Lee HW, Kim JW, Kim MK, Kwon GY, Han S, Kim SE, Kim SM, Chang SK
Digestive diseases and sciences 2014 May;59(5):1001-11
Digestive diseases and sciences 2014 May;59(5):1001-11
Activation of cutaneous immune responses in complex regional pain syndrome.
Birklein F, Drummond PD, Li W, Schlereth T, Albrecht N, Finch PM, Dawson LF, Clark JD, Kingery WS
The journal of pain 2014 May;15(5):485-95
The journal of pain 2014 May;15(5):485-95
Regional Differences in Chronic Stress-induced Alterations in Mast Cell and Protease-activated Receptor-2-positive Cell Numbers in the Colon of Ws/Ws Rats.
Kim YS, Lee MY, Ryu HS, Choi ES, Oh JT, Yun KJ, Choi SC
Journal of neurogastroenterology and motility 2014 Jan;20(1):54-63
Journal of neurogastroenterology and motility 2014 Jan;20(1):54-63
The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
Rusu MC, Didilescu AC, Stănescu R, Pop F, Mănoiu VM, Jianu AM, Vâlcu M
Anatomical record (Hoboken, N.J. : 2007) 2013 Feb;296(2):350-63
Anatomical record (Hoboken, N.J. : 2007) 2013 Feb;296(2):350-63
Histamine induces the neuronal hypertrophy and increases the mast cell density in gastrointestinal tract.
Keles N, Yavuz Arican R, Coskun M, Elpek GO
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2012 Nov;64(7-8):713-6
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2012 Nov;64(7-8):713-6
Pressure overload leads to an increase of cardiac resident stem cells.
Rupp S, Bauer J, von Gerlach S, Fichtlscherer S, Zeiher AM, Dimmeler S, Schranz D
Basic research in cardiology 2012 Mar;107(2):252
Basic research in cardiology 2012 Mar;107(2):252
Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps.
Peterson S, Poposki JA, Nagarkar DR, Chustz RT, Peters AT, Suh LA, Carter R, Norton J, Harris KE, Grammer LC, Tan BK, Chandra RK, Conley DB, Kern RC, Schleimer RP, Kato A
The Journal of allergy and clinical immunology 2012 Jan;129(1):119-27.e1-9
The Journal of allergy and clinical immunology 2012 Jan;129(1):119-27.e1-9
Soluble mediators released from PI-IBS patients' colon induced alteration of mast cell: involvement of reactive oxygen species.
Han W, Lu X, Jia X, Zhou T, Guo C
Digestive diseases and sciences 2012 Feb;57(2):311-9
Digestive diseases and sciences 2012 Feb;57(2):311-9
Identification of mast cells and mast cell subpopulations.
Buckley M, Walls AF
Methods in molecular medicine 2008;138:285-97
Methods in molecular medicine 2008;138:285-97
Immediate anaphylactic death following antibiotics injection: splenic eosinophilia easily revealed by pagoda red stain.
Trani N, Bonetti LR, Gualandri G, Barbolini G
Forensic science international 2008 Oct 25;181(1-3):21-5
Forensic science international 2008 Oct 25;181(1-3):21-5
Postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents: evaluation of clinical laboratory parameters and immunohistochemistry in three cases.
Osawa M, Satoh F, Horiuchi H, Tian W, Kugota N, Hasegawa I
Legal medicine (Tokyo, Japan) 2008 May;10(3):143-7
Legal medicine (Tokyo, Japan) 2008 May;10(3):143-7
Interstitial Cajal-like cells in rat mesentery: an ultrastructural and immunohistochemical approach.
Hinescu ME, Popescu LM, Gherghiceanu M, Faussone-Pellegrini MS
Journal of cellular and molecular medicine 2008 Jan-Feb;12(1):260-70
Journal of cellular and molecular medicine 2008 Jan-Feb;12(1):260-70
Elastofibroma: a clinicopathologic and immunohistochemical study of seven cases and literature review.
Gun BD, Bahadir B, Behzatoglu K, Gun MO, Ozdamar SO
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2007 Feb;115(2):115-9
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2007 Feb;115(2):115-9
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Popescu LM, Gherghiceanu M, Hinescu ME, Cretoiu D, Ceafalan L, Regalia T, Popescu AC, Ardeleanu C, Mandache E
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Popescu LM, Gherghiceanu M, Hinescu ME, Cretoiu D, Ceafalan L, Regalia T, Popescu AC, Ardeleanu C, Mandache E
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.
Cusi MG, Del Vecchio MT, Terrosi C, Savellini GG, Di Genova G, La Placa M, Fallarino F, Moser C, Cardone C, Giorgi G, Francini G, Correale P
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7210-6
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7210-6
Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.
Cusi MG, Del Vecchio MT, Terrosi C, Savellini GG, Di Genova G, La Placa M, Fallarino F, Moser C, Cardone C, Giorgi G, Francini G, Correale P
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7210-6
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7210-6
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with Mast Cell Tryptase antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Characterization of mast cells derived from bone marrow CD34+ hematopoietic precursors. A. Immunofluorescence staining of original CD34+ hematopoietic precursors and differentiated mast cells. Anti-tryptase antibody or anti-chymase antibody was used in the experiments. DAPI was used to stain the nucleus of the cells. B. Quantification of experiments described in A, more than 200 cells were counted in each group, mean+-SD, n = 3. C. Toluidine blue staining of the derived mast cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 MIF knockdown inhibits the profibrogenic activity of Mast cells. A and B. The mRNA and protein levels of MIF in mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. C. Western-blots of chymase, tryptase and GAPDH in original or mast cells (derived from CD34+ hematopoietic precursors with or without MIF knockdown). D. Toluidine blue staining of the mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. E. Quantification analysis of FcepsilonRI/CD117 double positive population in original or mast cells (derived from CD34+ hematopoietic precursors with or without MIF knockdown), #P>0.05. F. Histamine release assay of the mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. G and H. The growth curves and collagen synthesis of fibroblast cultured in medium conditioned by mast cells with or without MIF knockdown, mean+-SD, n = 3.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Representative slides of IHC staining with anti-FCERI and anti-TPSAB1 to visualize mast cells in osteophyte sections. Representative areas of osteophytes of study patients are shown as P1, P2 and P3. Antibody-stained mast cells were localized in bone trabeculae, cartilage region, where the columnar chondrocytes can be seen. MC are mast cells at 100X magnification revealing granular features. Bottom panel: histograms--control and osteophytes, denote anti-FCERI and anti-TPSAB1 stained cells (%) respectively; * p < 0.05, ** p < 0.01 compared to control.
