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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- 21619-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FHL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTERFDCHH CNESLFGKKY ILREESPYCV VCFETLFANT CEECGKPIGC DCKDLSYKDR HWHEACFHCS QCRNSLVDKP FAAKEDQLLC TDCYSNEYSS KCQECKKTIM PGTRKMEYKG SSWHETCFIC HRCQQPIGTK SFIPKDNQNF CVPCYEKQHA MQCVQCKKPI TTGGVTYREQ PWHKECFVCT ACRKQLSGQR FTARDDFAYC LNCFCDLYAK KCAGCTNPIS GLGGTKYISF EERQWHNDCF NCKKCSLSLV GRGFLTERDD ILCPDCGKDI (1-279 aa encoded by BC014397)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.
