MA5-42563

antibody from Invitrogen Antibodies

Targeting: ESYT2 CHR2SYT, FAM62B, KIAA1228

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42563

Provider

Invitrogen Antibodies

Product name

FAM62B Monoclonal Antibody (C4-D0)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Siha cell lysate, THP-1 cell lysate, human colon carcinoma tissue.

Antibody clone number

C4-D0

Concentration

2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FAM62B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. FAM62B Monoclonal Antibody (Product # MA5-42563) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Siha cell lysate. Lane 2: THP-1 cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human colon carcinoma tissue using FAM62B Monoclonal Antibody (Product # MA5-42563). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the FAM62B antibody at a dilution of 1:100 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.